AAV with CamKII promoter driven Venus

This AAV expresses Venus driven by a neuron CamKII promoter.

This 1.3 Kb promoter is derived from the murine α-Calcium/calmodulin-dependent kinase II (CaMKII), a gene with expression restricted to excitatory neurons in the neocortex and hippocampus. In cortical neurons, it was shown that the promoter activity of CaMKII(1.3) is about double that of CaMKII(2.4), probably due to some inhibitory element(s).

Like mCitrine and EYFP, Venus was developed in an effort to get better variants of YFP in terms of brightness and photo-stability. Venus has an Excitation/Emission spectrum of 515nm/529nm which is almost identical to that of EYFP and mCitrine.