AAV with CamKII promoter driven EBFP2

This AAV expresses EBFP2 driven by a neuron CamKII promoter.

This 1.3 Kb promoter is derived from the murine α-Calcium/calmodulin-dependent kinase II (CaMKII), a gene with expression restricted to excitatory neurons in the neocortex and hippocampus. In cortical neurons, it was shown that the promoter activity of CaMKII(1.3) is about double that of CaMKII(2.4), probably due to some inhibitory element(s).

EBFP2 is new version of blue fluorescent protein (BFP), with an Excitation/Emission spectrum of 380nm/448nm. EBFP2 is the brightest and most photo-stable of the BFPs; it is useful for situations in which a blue probe is required, especially when two-photon excitation is used