AAV with ELA1 promoter driven fLuc

This AAV expresses fLuc driven by a pancreatic acinar ELA1 promoter.

Rat elastase 1 (Ela1; ELAI1; RATELAI1), also called chymotrypsin-like elastase family, member 1, is the major protease with elastin-degrading activity in the mammalian exocrine pancreas. The ELA1 promoter (0.3Kb) is comprised of the core promoter and enhancer, and directs transgene expression in pancreatic acinar cells, stomach, duodenum, and colon.

Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.