AAV with hRPE(0.8) promoter driven EGFP

This AAV expresses EGFP driven by a retina hRPE(0.8) promoter.

The RPE65 gene encodes a protein of the retinal pigment epithelium (RPE) with a key role in the cycling of retinoids. It was shown that a RPE promoter of about 0.8 Kb was sufficient to direct high RPE-specific expression in transgenic mice. The 1.5Kb version of the promoter hRPE(1.5), also gives RPE-specific expression, but the expression level is not as high as the 0.8Kb promoter. This is probably due to a negative regulatory element(s) present in the 1.5Kb promoter.

EGFP is derived from the Aequorea GFP with a Excitation/Emission spectrum around 488nm/507nm. It is the most commonly used imaging probe. Other fluorescent proteins with similar emission spectrums are Emerald, and T-Sapphire