AAV with eSYN promoter driven ECFP

This AAV expresses ECFP driven by a neuron eSYN promoter.

The eSYN is a hybrid promoter consisting of a 0.4Kb CMV enhancer (E) and the 0.45Kb human Synapsin I promoter fragment (hSYN1). eSYN hybrid promoter gives about 2-4 fold higher expression than that of the parental hSYN1 promoter. However, the neuronal specificity of eSYN is slightly decreased, compared to that of the parental hSYN1 promoter.

Enhanced Cyan fluorescence protein(ECFP) is a cyan variant from the Aequorea GFP, with a Excitation/Emission spectrum around 434nm/477nm. Other versions of fluorescent proteins with similar excitation and emission spectrums include mcerulean3, mTurquoise, and SCFP