AAV with EF1a promoter driven roGFP1

This AAV expresses roGFP1 driven by an ubiquitous EF1a promoter.

The 1.2 Kb EF1α promoter is a commonly used, constitutive promoter derived from human elongation factor-1 alpha (EF1α). It is often used in conditions where the other constitutive promoters, such as CMV, have diminished activity or have been silenced.

roGFP1 is a reduction-oxidation sensitive green fluorescent protein used as a biosensor. Two cysteines were introduced into the beta-barrel structure of GFP; the oxidation state, reduced dithiol or the oxidized disulfide, of the engineered thiols determines the fluorescent properties of the sensor. roGFPs (roGFP1 and roGFP2), have two fluorescent excitation maxima at about 400 and 490 nm, respectively and display rapid and reversible ratiometric changes in fluorescence in response to changes in redox potential in vitro and in vivo.