AAV with MBP promoter driven eNpHR3.0-ECFP

This AAV expresses eNpHR3.0-ECFP driven by an olgiodendricyte MBP promoter.

The myelin basic proteins (MBPs) are a family of polypeptides that are predominantly expressed in the nervous system, where they play a major role in myelination. This 1.3 Kb MBP promoter drives reporter gene expression mainly in oligodendrocytes.

NpHR is a halorhodopsin or a light-sensitive chloride channel that provides silencing of neuronal activity by hyperpolarization. It offers fast inhibition, and its peak activation falls at 589 nm.

The trafficking signal (KSRITSEGEYIPLDQIDINV) is also from the inward rectifier potassium channel Kir2.1; it serves to dramatically reduce intracellular accumulation and improve membrane targeting, leading to a profound increase of photocurrents. This is the basis for many third-generation optogenetics tools

This ER export motif (FCYENEV) is from the vertebrate inward rectifier potassium channel Kir2.1. The motif prevents aggregate formation, decreases intracellular accumulations, and enhances tolerability at high expression levels. This is the strategy for developing many second-generation optogenetics tools