AAV with CMV-IVS promoter driven C1V1(E162T)-TS-ECFP

This AAV expresses C1V1(E162T)-TS-ECFP driven by an ubiquitous CMV-IVS promoter.

The CMV-IVS promoter is about 1.1 kb in length and consists of the commonly used CMV promoter/enhancer and the intron (IVS) from beta-globin . The presence of intron elements was shown to give a higher level of transgene expression than that of the original CMV promoter/enhancer.

C1V1 is a chimeric channelrhodopsin made to boost membrane expression higher than VChR1 but with a different light spectrum. It is composed of ChR1 and VChR1 fragments, and implements fast, potent optical excitation at red-shifted wavelengths. C1V1(E162T) mutant has an off-kinetic is half of C1V1.

The trafficking signal (KSRITSEGEYIPLDQIDINV) is also from the inward rectifier potassium channel Kir2.1; it serves to dramatically reduce intracellular accumulation and improve membrane targeting, leading to a profound increase of photocurrents. This is the basis for many third-generation optogenetics tools