AAV with CamKII(0.4) promoter driven hChR2(E123T/T159C)-EGFP

This AAV expresses hChR2(E123T/T159C)-EGFP driven by a neuron CamKII(0.4) promoter.

This 0.4 Kb promoter is derived from murine α-Calcium/calmodulin-dependent kinase II (CaMKII), a gene with expression restricted to excitatory neurons in the neocortex and hippocampus. It was shown that the promoter activity of CaMKII(0.4) is almost identical to that of CaMKII(1.3) in cortical neurons, Compared to CaMKII(1.3), this short promoter allows cloning of longer gene of interest into the AAV construct .

hChR2 is a humanized version of ChR2 for mammalian expression. Wild-type ChR2, as well as a few of its mutations, provides the fastest excitation of the channelrhodopsins offered, and is widely used in in optogenetics techniques in neuroscience. The hChR2(E123T/T159C) double mutation combines larger photocurrents from the T159C mutation and faster kinetics from the E123T mutation showing the highest speed/photocurrent combination so far. It is referred to as the second generation ultrafast optogenetics control.