AAV with CMV promoter driven hChR2(E123T)-mCitrine
Cat. No: VB3177
Availability:
2-3 weeks
Name:
AAV-cDNA6-hChR2(E123T)-mCitrine
This AAV expresses hChR2(E123T)-mCitrine driven by an ubiquitous CMV promoter.
This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).
hChR2 is a humanized version of ChR2 for mammalian expression. Wild-type ChR2, as well as a few of its mutations, provides the fastest excitation of the channelrhodopsins offered, and is widely used in in optogenetics techniques in neuroscience. hChR2(E123T) mutant accelerates the open state decay but reduces photocurrent amplitudes. Its maximal spectrum is near 495nM.
This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).
hChR2 is a humanized version of ChR2 for mammalian expression. Wild-type ChR2, as well as a few of its mutations, provides the fastest excitation of the channelrhodopsins offered, and is widely used in in optogenetics techniques in neuroscience. hChR2(E123T) mutant accelerates the open state decay but reduces photocurrent amplitudes. Its maximal spectrum is near 495nM.
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Viral Details
- Viral Backbone
- Recombinant AAV
- AAV-ITR
- AAV2
- AAV Serotype
- Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
- Promoter
- CMV (ubiquitous)
- Storage Buffer
- PBS/5% Glycerol
- Volume
- 200ul
- Titer
- 1x10^13 GC/ml
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