AAV with CMV promoter driven hChR2(E123T/T159C)-EGFP

This AAV expresses hChR2(E123T/T159C)-EGFP driven by an ubiquitous CMV promoter.

This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).

hChR2 is a humanized version of ChR2 for mammalian expression. Wild-type ChR2, as well as a few of its mutations, provides the fastest excitation of the channelrhodopsins offered, and is widely used in in optogenetics techniques in neuroscience. The hChR2(E123T/T159C) double mutation combines larger photocurrents from the T159C mutation and faster kinetics from the E123T mutation showing the highest speed/photocurrent combination so far. It is referred to as the second generation ultrafast optogenetics control.