AAV with CAG2 promoter driven hChR2(E123T/H134R)-EYFP
Cat. No: VB2988
Availability:
2-3 weeks
Name:
AAV-CAG2-hChR2(E123T/H134R)-EYFP
This AAV expresses hChR2(E123T/H134R)-EYFP driven by an ubiquitous CAG2 promoter.
The CAG2 promoter is derived from the commonly used 1.8kb CAG/CBA (also called CAGGS) promoter. To increase the cloning capacity of size-limiting vectors using the CAG promoter, such as AAV or lentiviral vectors, CAG2 was developed by deleting a portion of about 0.6Kb of the chicken beta-actin intron from the original CAG promoter.
hChR2(E123T/H134R), also known as ChETA, is a channelrhodopsin-2 variant with faster deactivation kinetics.
The CAG2 promoter is derived from the commonly used 1.8kb CAG/CBA (also called CAGGS) promoter. To increase the cloning capacity of size-limiting vectors using the CAG promoter, such as AAV or lentiviral vectors, CAG2 was developed by deleting a portion of about 0.6Kb of the chicken beta-actin intron from the original CAG promoter.
hChR2(E123T/H134R), also known as ChETA, is a channelrhodopsin-2 variant with faster deactivation kinetics.
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Viral Details
- Viral Backbone
- Recombinant AAV
- AAV-ITR
- AAV2
- AAV Serotype
- Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
- Promoter
- CAG2 (ubiquitous)
- Storage Buffer
- PBS/5% Glycerol
- Volume
- 200ul
- Titer
- 1x10^13 GC/ml
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