AAV with ApoE/hAAT1 promoter driven TdTomato

This AAV expresses TdTomato driven by a liver ApoE/hAAT1 promoter.

The ApoE/AAT1 chimeric promoter consists of the core promoter from human alpha-1-antitrypsin gene (also known as hAAT1, SERPINA1, A1A, A1AT, AAT, or alpha1AT) and the enhancer/hepatic locus control region (HCR) from the ApoE gene. The ApoE-hAAT1 chimeric promoter is about 0.7Kb and is used for driving gene expression in hepatocytes

dTomato belongs to a family of fluorescent proteins called mFruit. Two dTomato units were joined head-to-tail through a linker, yielding a tandem dimer fluorescent protein, tdTomato. The tdTomato derivative is the brightest fluorescent protein yet reported, featuring an orange-red emission maximum at 581nm and is one of the most photo-stable fluorescent proteins. Other fluorescent proteins with similar emission spectra include DsRed, DsRed2, TurboRFP, TagRFP, and TagRFP-T.