AAV with SST promoter driven Mito-roGFP1

This AAV expresses Mito-roGFP1 driven by an islet SST promoter.

The rat SST gene (also known as SS-14, SS-28, or Smst) is synthesized in the pancreatic islets, gastrointestinal tract, nervous system, and thyroid gland. The promoter (SSTp) is a 0.9Kb regulatory fragment from the rat SST gene.

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.