AAV with SST promoter driven roGFP1

This AAV expresses roGFP1 driven by an islet SST promoter.

The rat SST gene (also known as SS-14, SS-28, or Smst) is synthesized in the pancreatic islets, gastrointestinal tract, nervous system, and thyroid gland. The promoter (SSTp) is a 0.9Kb regulatory fragment from the rat SST gene.

roGFP1 is a reduction-oxidation sensitive green fluorescent protein used as a biosensor. Two cysteines were introduced into the beta-barrel structure of GFP; the oxidation state, reduced dithiol or the oxidized disulfide, of the engineered thiols determines the fluorescent properties of the sensor. roGFPs (roGFP1 and roGFP2), have two fluorescent excitation maxima at about 400 and 490 nm, respectively and display rapid and reversible ratiometric changes in fluorescence in response to changes in redox potential in vitro and in vivo.