AAV with ELA1 promoter driven TdTomato

This AAV expresses TdTomato driven by a pancreatic acinar ELA1 promoter.

Rat elastase 1 (Ela1; ELAI1; RATELAI1), also called chymotrypsin-like elastase family, member 1, is the major protease with elastin-degrading activity in the mammalian exocrine pancreas. The ELA1 promoter (0.3Kb) is comprised of the core promoter and enhancer, and directs transgene expression in pancreatic acinar cells, stomach, duodenum, and colon.

dTomato belongs to a family of fluorescent proteins called mFruit. Two dTomato units were joined head-to-tail through a linker, yielding a tandem dimer fluorescent protein, tdTomato. The tdTomato derivative is the brightest fluorescent protein yet reported, featuring an orange-red emission maximum at 581nm and is one of the most photo-stable fluorescent proteins. Other fluorescent proteins with similar emission spectra include DsRed, DsRed2, TurboRFP, TagRFP, and TagRFP-T.