AAV with mIP1 promoter driven fLuc

This AAV expresses fLuc driven by an islet mIP1 promoter.

The mouse insulin-I promoter, (m)INS1, mIP-I or mIP1, is a promoter fragment of about 1.2 kb in length from the murine preproinsulin-I gene. It drives transgene expression in beta- cells of the pancreas, in the research field of diabetes and obesity.

Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.