AAV with mIP1 promoter driven TdTomato

This AAV expresses TdTomato driven by an islet mIP1 promoter.

The mouse insulin-I promoter, (m)INS1, mIP-I or mIP1, is a promoter fragment of about 1.2 kb in length from the murine preproinsulin-I gene. It drives transgene expression in beta- cells of the pancreas, in the research field of diabetes and obesity.

dTomato belongs to a family of fluorescent proteins called mFruit. Two dTomato units were joined head-to-tail through a linker, yielding a tandem dimer fluorescent protein, tdTomato. The tdTomato derivative is the brightest fluorescent protein yet reported, featuring an orange-red emission maximum at 581nm and is one of the most photo-stable fluorescent proteins. Other fluorescent proteins with similar emission spectra include DsRed, DsRed2, TurboRFP, TagRFP, and TagRFP-T.