AAV with ALB(1.4) promoter driven mCitrine

This AAV expresses mCitrine driven by a liver ALB(1.4) promoter.

The ALB(1.4) is a shorter version of the ALB(1.7) promoter with a total length of 1.4 Kb. In mouse liver cells, the transgene expression from this ALB(1.4) synthetic promoter is about 3 times lower than that of ALB(1.7) promoter but 10-100 fold greater than that of the CMV promoter 10-20 weeks after plasmid injection into mouse liver.

mCitrine, like Venus, is derived from YFP, with increased brightness and stability. mCitrine has an Excitation/Emission spectrum of 516nm/529nm which is almost identical to that of EYFP, Venus and Topaz.