AAV with ALB(1.7) promoter driven Mito-roGFP1

This AAV expresses Mito-roGFP1 driven by a liver ALB(1.7) promoter.

The ALB(1.7) is a shorter version of the ALB(1.9) promoter with a total length of 1.7 Kb. In mouse liver cells, the transgene expression from this ALB(1.7) synthetic promoter is about 3-5 times lower than that of the ALB(1.9) promoter but 20-200 fold greater than that of the CMV promoter 10-20 weeks after plasmid injection into mouse liver.

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.