AAV with CMV7 promoter driven SEAP

This AAV expresses SEAP driven by an ubiquitous CMV7 promoter.

The CMV7 promoter is about 0.35 kb in length and is a shortened version of the commonly used CMV promoter. CMV7 has a truncated version of the CMV enhancer. When tested in several cell lines, this promoter provides an expression level that is about 3-5 fold lower than that of the original 0.6Kb CMV promoter.

The SEAP reporter gene encodes a truncated form of the human placental alkaline phosphatase that lacks the membrane anchoring domain. Therefore, the protein can be efficiently secreted from transfected cells allowing for detection of reporter gene activity without cell lysis. Using a secreted reporter protein has several advantages over traditional reporter assays: 1) Cell lysis is not required for analysis so a single set of cells can be used for both the SEAP assay and another purpose; 2) Gene expression kinetics can be studied by the repeated collection of the culture medium from the same cultures; and 3) By changing the culture medium prior to an experiment, the assay background is reduced to an extremely low level.