AAV with CAG2 promoter driven EBFP2

This AAV expresses EBFP2 driven by an ubiquitous CAG2 promoter.

The CAG2 promoter is derived from the commonly used 1.8kb CAG/CBA (also called CAGGS) promoter. To increase the cloning capacity of size-limiting vectors using the CAG promoter, such as AAV or lentiviral vectors, CAG2 was developed by deleting a portion of about 0.6Kb of the chicken beta-actin intron from the original CAG promoter.

EBFP2 is new version of blue fluorescent protein (BFP), with an Excitation/Emission spectrum of 380nm/448nm. EBFP2 is the brightest and most photo-stable of the BFPs; it is useful for situations in which a blue probe is required, especially when two-photon excitation is used