AAV with GFAP(2.2) promoter driven roGFP1

This AAV expresses roGFP1 driven by an astrocyte GFAP(2.2) promoter.

The GFAP(2.2) promoter is based on human glial fibrillary acidic protein gene (GFAP), which is the major intermediate filament protein in astrocytes. It has been shown that approximately 2 kb of 5'-flanking DNA of the human GFAP gene is sufficient to direct transgene expression to astrocytes and confer developmental/injury-induced regulation.

roGFP1 is a reduction-oxidation sensitive green fluorescent protein used as a biosensor. Two cysteines were introduced into the beta-barrel structure of GFP; the oxidation state, reduced dithiol or the oxidized disulfide, of the engineered thiols determines the fluorescent properties of the sensor. roGFPs (roGFP1 and roGFP2), have two fluorescent excitation maxima at about 400 and 490 nm, respectively and display rapid and reversible ratiometric changes in fluorescence in response to changes in redox potential in vitro and in vivo.