mouse ATG4B shRNA silencing Adenovirus

Product Info

Knockdown

>90% knockdown of mRNA in cultured cells

Viral Details

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Promoter

U6

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Gene Details

Species

Mouse

Gene Symbol

Atg4b

Gene Name

autophagy related 4B, cysteine peptidase

Gene ID

66615

Gene Synonyms

2510009N07Rik; Apg4b; Atg4bl; Autl1; AW048066

ORF Size

1182 bp

RefSeq#

NM_174874

Gene Description

Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

UniGene ID

Mm.29087