human MTHFD1 shRNA silencing Adenovirus

Product Info

Knockdown

>90% knockdown of mRNA in cultured cells

Viral Details

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Promoter

U6

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Gene Details

Species

Human

Gene Symbol

MTHFD1

Gene Name

methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1

Gene ID

4522

Gene Synonyms

MTHFC; MTHFD

ORF Size

2808 bp

RefSeq#

NM_005956

Gene Description

MTHFD1 encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

UniGene ID

Hs.652308

HGNC ID

HGNC:7432