human DPH1 shRNA silencing Adenovirus

Product Info

Knockdown

>90% knockdown of mRNA in cultured cells

Viral Details

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Promoter

U6

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Gene Details

Species

Human

Gene Symbol

DPH1

Gene Name

diphthamide biosynthesis 1

Gene ID

1801

Gene Synonyms

DEDSSH; DPH2L; DPH2L1; OVCA1

ORF Size

912 bp

RefSeq#

NM_001383

Gene Description

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 (PubMed 15485916)).(supplied by OMIM, Mar 2008)

UniGene ID

Hs.513856

HGNC ID

HGNC:3003