Cre Recombinase Adenovirus
Cat. No: 1700
Availability:
Immediate
Name:
Ad-Cre-GFP
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.
This adenovirus expresses both Cre recombinase and GFP as marker. Cre and GFP are driven by 2 separate promoters.
Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).
$475.00
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Viral Details
- Viral Backbone
- Human Adenovirus Type5 (dE1/E3)
- Promoter
- CMV (ubiquitous)
- Reporter
- eGFP
- Storage Buffer
- DMEM, 2% BSA, 2.5% Glycerol
- Volume
- 200ul
- Titer
- 1x10^10 PFU/ml
Gene Details
- Gene Symbol
- Cre
Product Citations
This product is referenced in the following publications:
- Vittorio Sebastiano, etc (2011). In Situ Genetic Correction of the Sickle Cell Anemia Mutation in Human Induced Pluripotent Stem Cells Using Engineered Zinc Finger Nucleases. Stem Cells.
- Kon, N. etc (2011). Inactivation of ARF-BP1 induces p53 activation and the diabetic phenotypes in mice. J Biol Chem.
- Augustine Rajakumar, etc (2012). Transcriptionally Active Syncytial Aggregates in the Maternal Circulation May Contribute to Circulating Soluble Fms-Like Tyrosine Kinase 1 in Preeclampsia. Hypertension.
- Smith, MK. etc (2012). DIRECT AND INDIRECT ROLES FOR ¿-CATENIN IN FACULTATIVE BASAL PROGENITOR CELL DIFFERENTIATION. American Journal of Physiology.
- Kristin A. Anderson, etc (2012). Deletion of CaMKK2 from the Liver Lowers Blood Glucose and Improves Whole-Body Glucose Tolerance in the Mouse. Molecular Endocrinology.
- Mian, I. etc (2012). LKB1 Destabilizes Microtubules in Myoblasts and Contributes to Myoblast Differentiation. PLoS ONE.
- Aditya Murthy, etc (2012). Notch Activation by the Metalloproteinase ADAM17 Regulates Myeloproliferation and Atopic Barrier Immunity by Suppressing Epithelial Cytokine Synthesis. Immunity.
- Scotti ML, etc (2012). Protein Kinase C Iota Regulates Pancreatic Acinar-to-Ductal Metaplasia. PLoS ONE.
- Sabbir, MG, etc (2012). The Role of Dlc1 Isoform 2 in K-Ras2-G12D Induced Thymic Cancer. PLoS ONE.
- Refsland, EW, etc (2012). Endogenous Origins of HIV-1 G-to-A Hypermutation and Restriction in the Nonpermissive T Cell Line CEM2n. PLoS Pathogens.
- Zhang R, etc (2012). Lipasin, a novel nutritionally-regulated liver-enriched factor that regulates serum triglyceride levels. Biochem Biophys Res Commun.
- Proffitt KD, etc (2012). Precise Regulation of Porcupine Activity is Required for Physiological Wnt Signaling. J Biol Chem.
- X.-J. Qin, etc (2012). Protein tyrosine phosphatase SHP2 regulates TGF-ß1 production in airway epithelia and asthmatic airway remodeling in mice. Allergy.
- Janine Ehser, etc (2012). Molecular mimicry rather than identity breaks T-cell tolerance in the CYP2D6 mouse model for human autoimmune hepatitis. Journal of Autoimmunity.
- Ayesha Zafir, etc (2013). Protein O-GlcNAcylation is a Novel Cytoprotective Signal in Cardiac Stem Cells. Stem Cells.
- Roy F. Collaco, etc (2009). Adeno-Associated Virus and Adenovirus Coinfection Induces a Cellular DNA Damage and Repair Response via Redundant Phosphatidylinositol 3-Like Kinase Pathways. Virology.
- Albers, J. etc (2013). Combined mutation of Vhl and Trp53 causes renal cysts and tumours in mice. EMBO Molecular Medicine.
- Zhou J. etc (2013). Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis. Journal of Molecular and Cellular Cardiology.
- Osborn, MJ. etc (2013). TALEN-based Gene Correction for Epidermolysis Bullosa. Molecular Therapy.
- Guohui Wan, etc (2013). DNA-Damage-Induced Nuclear Export of Precursor MicroRNAs Is Regulated by the ATM-AKT Pathway. Cell Reports.
- Andrew Varble (2013). An In Vivo RNAi Screening Approach to Identify Host Determinants of Virus Replication. Cell Host & Microbe.
- Alice H. Huang (2013). Repositioning Forelimb Superficialis Muscles: Tendon Attachment and Muscle Activity Enable Active Relocation of Functional Myofibers. Developmental Cell.
- Sara Cogliati (2013). Mitochondrial Cristae Shape Determines Respiratory Chain Supercomplexes Assembly and Respiratory Efficiency. Cell.
- Olszewski MB, etc (2013). Disruption of clathrin-mediated trafficking causes centrosome overduplication and senescence. Traffic.
- Li Liu, etc (2013). Histone Demethylase LSD1 is Involved in SALL4 Mediated Transcriptional Repression in Hematopoietic Stem Cells. The Journal of Biological Chemistry.
- You-Soub Lee (2013). Runx3 Inactivation Is a Crucial Early Event in the Development of Lung Adenocarcinoma. Cancer Cell.
- Li Liu (2013). Histone Lysine-specific Demethylase 1 (LSD1) Protein Is Involved in Sal-like Protein 4 (SALL4)-mediated Transcriptional Repression in Hematopoietic Stem Cells. The Journal of Biological Chemistry.
- Matsumoto K. etc (2010). Transcription Coactivator Pbp/Med1 Deficient Hepatocytes Are Not Susceptible To Diethylnitrosamine-Induced Hepatocarcinogenesis In The Mouse. Carcinogenesis.
- Christian Beyer, etc (2014). Stimulation of the soluble guanylate cyclase (sGC) inhibits fibrosis by blocking non-canonical TGFß signalling. Ann Rheumatic Diseases.
- De He, etc (2014). MiR-371-5p Facilitates Pancreatic Cancer Cell Proliferation and Decreases Patient Survival. PLOS One.
- AT Henze, etc (2014). Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR. Nature Communications.
- MA Levy, etc (2014). ATRX promotes gene expression by facilitating transcriptional elongation through guanine-rich coding regions. Human Molecular Genetics.
- MJ Osborn, etc (2014). Fanconi Anemia Gene Editing by the CRISPR/Cas9 System. Human Gene Therapy.
- Grzegorz Sarek, etc (2015). TRF2 Recruits RTEL1 to Telomeres in S Phase to Promote T-Loop Unwinding. Molecular Cell.
- Pietro, N. etc (2010). Serum- And Glucocorticoid-Inducible Kinase 1 (Sgk1) Regulates Adipocyte Differentiation Via Forkhead Box O1. Molecular Endocrinology.
- MA A, etc (2015). Transcriptional modulator ZBED6 affects cell cycle and growth of human colorectal cancer cells. PNAS.
- Madhuri Kalathur, etc (2015). A chemogenomic screening identifies CK2 as a target for pro-senescence therapy in PTEN-deficient tumours. Nature Communications.
- A Zafir, etc (2015). O-GlcNAcylation Negatively Regulates Cardiomyogenic Fate in Adult Mouse Cardiac Mesenchymal Stromal Cells. PLoS ONE.
- Wu LA, etc (2015). Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells is Essential for Study of Osteoblast Growth, Differentiation and Osteogenesis. Journal of Cellular Physiology.
- E Raemy, etc (2016). Cardiolipin or MTCH2 can serve as tBID receptors during apoptosis. Cell Death & Differentiation.
- T Sugar, etc (2016). N-sulfation of heparan sulfate is critical for syndecan-4 mediated podocyte cell-matrix interactions. American Journal of Physiology.
- Désirée Schönenberger, etc (2016). Formation of renal cysts and tumors in Vhl/Trp53-deficient mice requires HIF-1a and HIF-2a. Cancer Research.
- Joseph E. Druso, etc (2016). An Essential Role for Cdc42 in the Functioning of the Adult Mammary Gland. JBC.
- Kiana Mahdaviani, etc (2016). Autocrine effect of vascular endothelial growth factor-A is essential for mitochondrial function in brown adipocytes. Metabolism.
- Hua Su (2009). Reduced Expression of Integrin {alpha} v {beta} 8 Is Associated with Brain Arteriovenous Malformation Pathogenesis. American Journal of Pathology.
- Shoujun Chen, etc (2010). Podocytes require the engagement of cell surface heparan sulfate proteoglycans for adhesion to extracellular matrices. Kidney International.
- Depner C, etc (2016). EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance. Nature Communications.
- Glytsou C, etc (2016). Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control. Cell Reports.
- Davis H, etc (2017). Disruption of the Cx43/miR21 pathway leads to osteocyte apoptosis and increased osteoclastogenesis with aging. Aging Cell.
- http://onlinelibrary.wiley.com/doi/10.1002/jbmr.3152/full (2017). Connexin43 and Runx2 Interact to Affect Cortical Bone Geometry, Skeletal Development, and Osteoblast and Osteoclast Function. Journal of Bone and Mineral Research.
- Fan W, etc (2017). PPARd Promotes Running Endurance by Preserving Glucose. Cell Metabolism.
- P Margalef, etc (2017). Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell.
- JF Mir, etc (2018). Ghrelin Causes a Decline in GABA Release by Reducing Fatty Acid Oxidation in Cortex. Molecular Neurobiology.
- G Greicius, etc (2018). PDGFRa+ pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo. PNAS.
- J Sim, etc (2018). The Factor Inhibiting HIF Asparaginyl Hydroxylase Regulates Oxidative Metabolism and Accelerates Metabolic Adaptation to Hypoxia. Cell Metabolism.
- O'Flaherty L, etc (2010). Dysregulation of hypoxia pathways in fumarate hydratase-deficient cells is independent of defective mitochondrial metabolism. Human Molecular Genetics.
- Pietro ND, etc (2010). Serum-and glucocorticoid-inducible kinase 1 (SGK1) regulates adipocyte differentiation via forkhead box O1. Molecular Endocrinology.
- Küste K, etc (2010). Downregulation of the coxsackie and adenovirus receptor in cancer cells by hypoxia depends on HIF-1a. Cancer Gene Therapy.
- D Soni, etc (2018). Deubiquitinase function of A20 maintains and repairs endothelial barrier after lung vascular injury. Cell Death Discovery.
- Dudakovic A, etc (2018). Enhancer of zeste homolog 2 (Ezh2) controls bone formation and cell cycle progression during osteogenesis in mice. JBC.
- Harashima A, etc (2010). Phosphorylation of the NFAR proteins by the dsRNA-dependent protein kinase PKR constitutes a novel mechanism of translational regulation and cellular defense. Genes & Development.
- M Tsuda, etc (2018). The BRG1/SOX9 axis is critical for acinar cell–derived pancreatic tumorigenesis. JCI.
- AB Khalid, etc (2018). GATA4 represses RANKL in osteoblasts via multiple long-range enhancers to regulate osteoclast differentiation. Bone.
- Daynac M, etc (2018). Article | OPEN | Published: 21 August 2018 Lgl1 controls NG2 endocytic pathway to regulate oligodendrocyte differentiation and asymmetric cell division and gliomagenesis. Nature Communications.
- EK Choi, etc (2018). Cyclin B1 stability is increased by interaction with BRCA1, and its overexpression suppresses the progression of BRCA1-associated mammary tumors. Experimental & Molecular Medicinevolume.
- D Zhou, etc (2018). Mammalian pigmentation is regulated by a distinct cAMP-dependent mechanism that controls melanosome pH. Sci. Signal.
- J Chen, etc (2019). Single-cell transcriptome analysis identifies distinct cell types and niche signaling in a primary gastric organoid model. Scientific Reports.
- Zukauskas A, etc (2011). TM4SF1: a tetraspanin-like protein necessary for nanopodia formation and endothelial cell migration. Angiogenesis.
- A Goring, etc (2019). Regulation of the bone vascular network is sexually dimorphic. Journal of Bone and Mineral Research.
- C Colomer, etc (2019). IKKa Kinase Regulates the DNA Damage Response and Drives Chemo-resistance in Cancer. Molecular Cell,.
- S Chen, etc (2019). Trimethylamine N-Oxide Binds and Activates PERK to Promote Metabolic Dysfunction. Cell Metabolism.
- G Sarek, etc (2019). CDK phosphorylation of TRF2 controls t-loop dynamics during the cell cycle. Nature.
- M Crawford, etc (2020). INTEGRIN-LINKED KINASE REGULATES MELANOSOME TRAFFICKING AND MELANIN TRANFER IN MELANOCYTES. Molecular Biology of the Cell.
- R Windmueller, etc (2020). Direct Comparison of Mononucleated and Binucleated Cardiomyocytes Reveals Molecular Mechanisms Underlying Distinct Proliferative Competencies. Cell Reports.
- S Chidambaram, etc (2020). Subventricular zone adult mouse neural stem cells and human glioblastoma stem cells require insulin receptor for self-renewal. bioRxiv.
- Guria, A. etc (2011). Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts. RNA.
- N Gudekar, etc (2020). Metallothioneins regulate ATP7A trafficking and control cell viability during copper deficiency and excess. Scientific Reports.
- R Bellelli, etc (2020). Synthetic Lethality between DNA Polymerase Epsilon and RTEL1 in Metazoan DNA Replication. Cell Reports.
- Mancin A, etc (2010). THOC5/FMIP, an mRNA export TREX complex protein, is essential for hematopoietic primitive cell survival in vivo. BMC Biology.
- C Papin, etc (2020). CpG Islands Shape the Epigenome Landscape. Journal of Molecular Biology.
- Sandra Schreiber, etc (2011). Phenotype of single hepatocytes expressing an activated version of ß-catenin in liver of transgenic mice. Journal of Molecular Histology.
- Ana Maria Gonzalez, etc (2011). Ecrg4 expression and its product augurin in the choroid plexus: impact on fetal brain development, cerebrospinal fluid homeostasis and neuroprogenitor cell response to CNS injury. CNS.
- Bielefeld KA, etc (2011). Fibronectin and {beta}-Catenin Act in a Regulatory Loop in Dermal Fibroblasts to Modulate Cutaneous Healing. J Biol Chem.
- Yang P, etc (2011). Role of HIF-1a in maternal hyperglycemia-induced embryonic vasculopathy. Am J Obstet Gynecol.
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