Shuttle Vectors for Adenovirus Construction

These are the most commonly used shuttle vectors for adenovirus construction.  To order any of the plasmids listed here, please request online or send us an email.

Adenoviral Shuttle Vectors for Gene Expression

For cDNA with no internal PacI sites:

  • DUAL-BASIC (map): empty shuttle, for cloning of your whole existing cassette
  • DUAL-CCM (map) & Dual-CCM(-): with CMV and polyA, for cloning of cDNA only, Dual-CCM(-) has the restriction sites inside MCS in exact reverse order.
  • DUAL2-CCM (map): with CMV and polyA, for cloning of cDNA only

Adenoviral Shuttle Vectors for shRNA/miRNA

For cloning from an existing shRNA/miRNA expression cassette:

  • DUAL-BASIC (map): empty shuttle, for transfer your existing shRNA/miRNA expression cassette

For cloning from an shRNA oligos

  • DUAL-U6 (map): the shRNA expression will be under the control of U6-promoter in this vector.
  • DUAL-Siln-MCS (map): shRNA expression will be under the control of a modified CMV promoter.

If you don’t the see the vectors you need here, please contact us for additional shuttle vector selections not listed here.