Ad-HT Adenovirus Amplification Service

Large Scale Adenovirus Amplification & CsCl Purification

This service is ideal for those who require recombinant adenovirus for in vivo studies or just require high-titer purified viruses.

First, we infect ~4000cm² of 293 cell monolayer through a series of amplifications from customer-provided viral stock or any adenovirus from our catalog. Then, once cytopathic effects begin to manifest, we harvest and lyse the cells to release the virus.  Next, the adenovirus is purified via centrifugation using two sequential cesium chloride gradients. The final product is desalted, then titered both spectrophotometrically for viral particles and via plaque formation assay for PFU/IFU.

Upon completion, you will receive a final viral stock of approximately 5×10¹² to 1×10¹³ viral particles (~1-3×10¹¹ PFU)*.  This process takes ~2-3 weeks to complete.

Medium Scale Adenovirus Amplification & Titration

This service is for those who require recombinant adenovirus for in vitro studies.

The process is similar to the large scale amplification. First, we infect ~1000cm² of HEK293 cell monolayer through a series of amplifications from customer-provided viral stock or any adenovirus from our catalog. Once cytopathic effects begin to manifest, we harvest and lyse the cells to release the virus. Then, the final product is titered for PFU/IFU.

Upon completion, you will receive a final viral stock of approximately 2ml with titer of 1-5×10¹⁰ PFU/IFU *.  This process takes ~2 weeks to complete.

* Final viral yield may depend on the transgene in the virus.