AAV ITR Vectors

We have developed over 15 different AAV ITR vectors for AAV production. All AAV plasmids here contain the L-ITR and R-ITR from AAV serotype 2 and are compatible with Helper Free AAV Systems. Below, you’ll find the most commonly used vectors. To order any of these plasmids, please request online or send us an email.

AAV Vectors for Gene Over-Expression

• pAAV-BASIC * (map): empty ITR vector, for cloning of whole existing cassettes.
• pAAV-BASIC-EGFP * (map): pAAV-BASIC, but with co-expression of eGFP.
• pAAV-cDNA6-V5His (map): with CMV promoter, for cloning of cDNA only.
• pAAV-GFP-cDNA6 (map): pAAV-cDNA6-V5His, but with co-expression of GFP.

AAV ITR Vectors for shRNA/miRNA

For cloning from an existing shRNA/miRNA expression cassette:

• pAAV-BASIC * (map): empty ITR vector, for cloning of whole existing cassettes
• pAAV-BASIC-EGFP * (map): pAAV-BASIC, but with co-expression of eGFP.

For cloning from an shRNA oligos:

• pAAV-U6 (map): shRNA expression under control of a U6-promoter.
• pAAV-U6-EGFP (map): pAAV-U6, but with co-expression of eGFP.
• pAAV-Siln41-MCS (map): shRNA expression under control of a modified CMV promoter.
• pAAV-Siln41-MCS-EGFP (map): pAAV-Siln41-MCS, but with co-expression of eGFP

* All GFP co-expression vectors are available with RFP as well.

If you don’t the see the vector you need here, please email us for additional AAV vector selections.