mouse EGLN2 shRNA silencing AAV

Cat. No: shAAV-258139

Availability: Immediate

Name: AAV-m-EGLN2-shRNA

This is an AAV expressing shRNA for silencing of Mouse Egln2.

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Viral Backbone: Recombinant AAV
AAV-ITR: AAV2
AAV Serotype: Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
Promoter: U6
Reporter: eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer: PBS/5% Glycerol

Species: Mouse
Gene Symbol: Egln2
Gene Name: egl-9 family hypoxia-inducible factor 2
Gene ID: 112406
Gene Synonyms: 0610011A13Rik; C85656; Hif-p4h-1; Ier4; Phd1; SM-20
ORF Size: 1260 bp
RefSeq#: NM_053208
Gene Description: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death.
UniGene ID: Mm.29978