mouse SF3B2 shRNA silencing Adenovirus

Product Info

Knockdown

>80% knockdown of mRNA in cultured cells

Viral Details

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Promoter

U6

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Gene Details

Species

Mouse

Gene Symbol

Sf3b2

Gene Name

splicing factor 3b, subunit 2

Gene ID

319322

Gene Synonyms

145kDa; 2610311M13Rik; 2810441F20Rik; B230398H18Rik; SAP145; SF3b1; SF3b145; SF3b150

ORF Size

2637 bp

RefSeq#

NM_030109

Gene Description

Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

UniGene ID

Mm.471551