AAV with ALB(1.0) promoter driven LacZ

This AAV expresses LacZ driven by a liver ALB(1.0) promoter.

The ALB(1.0) is a shorter version of the ALB(1.4) promoter with a total length of 1.0 Kb. In mouse liver cells, the transgene expression from this ALB(1.0) synthetic promoter is about 3-5 times lower than that of ALB(1.4) promoter but 10-20 fold greater than that of the CMV promoter 10-20 weeks after plasmid injection into mouse liver.

The bacterial enzyme -galactosidase (gene LacZ) is frequently used as a reporter gene. It can be easily located with LacZ staining using the artificial substrate X-gal, which turns blue when cleaved by -galactosidase (LacZ).