AAV with cTNT promoter driven Mito-roGFP1

This AAV expresses Mito-roGFP1 driven by a cardiac cTNT promoter.

cTNT promoter is a 0.4 Kb fragment derived from the chicken troponin T gene, and drives cardiomyocyte muscle-specific gene expression.

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.