AAV with aMHC promoter driven SEAP

This AAV expresses SEAP driven by a cardiac aMHC promoter.

αMHC(0.4) promoter is derived from the murine α-MHC gene (alpha-myosin heavy chain) and drives cardiac-specific expression. The length of this promoter is 0.4 Kb. Based on studies in primary cardiomyocytes, this aMHC(0.4) promoter gives an expression level that is comparable to that of a CMV promoter.

The SEAP reporter gene encodes a truncated form of the human placental alkaline phosphatase that lacks the membrane anchoring domain. Therefore, the protein can be efficiently secreted from transfected cells allowing for detection of reporter gene activity without cell lysis. Using a secreted reporter protein has several advantages over traditional reporter assays: 1) Cell lysis is not required for analysis so a single set of cells can be used for both the SEAP assay and another purpose; 2) Gene expression kinetics can be studied by the repeated collection of the culture medium from the same cultures; and 3) By changing the culture medium prior to an experiment, the assay background is reduced to an extremely low level.