AAV with ALB(1.0) promoter driven roGFP1

This AAV expresses roGFP1 driven by a liver ALB(1.0) promoter.

The ALB(1.0) is a shorter version of the ALB(1.4) promoter with a total length of 1.0 Kb. In mouse liver cells, the transgene expression from this ALB(1.0) synthetic promoter is about 3-5 times lower than that of ALB(1.4) promoter but 10-20 fold greater than that of the CMV promoter 10-20 weeks after plasmid injection into mouse liver.

roGFP1 is a reduction-oxidation sensitive green fluorescent protein used as a biosensor. Two cysteines were introduced into the beta-barrel structure of GFP; the oxidation state, reduced dithiol or the oxidized disulfide, of the engineered thiols determines the fluorescent properties of the sensor. roGFPs (roGFP1 and roGFP2), have two fluorescent excitation maxima at about 400 and 490 nm, respectively and display rapid and reversible ratiometric changes in fluorescence in response to changes in redox potential in vitro and in vivo.