AAV with ALB(1.7) promoter driven mCherry

This AAV expresses mCherry driven by a liver ALB(1.7) promoter.

The ALB(1.7) is a shorter version of the ALB(1.9) promoter with a total length of 1.7 Kb. In mouse liver cells, the transgene expression from this ALB(1.7) synthetic promoter is about 3-5 times lower than that of the ALB(1.9) promoter but 20-200 fold greater than that of the CMV promoter 10-20 weeks after plasmid injection into mouse liver.

mCherry was derived from DsRed through extensive mutagenesis. It has an Excitation/Emission spectrum of 590nm/610nm. mCherry's emission spectrum is similar to the following fluorescent markers: mRuby (Em=605nM), JRed (Em=610nM), mRFP1 (Em=607nM), and HcRed1