AAV with GFAP(0.7) promoter driven hChR2(E123A)-Venus

This AAV expresses hChR2(E123A)-Venus driven by an astrocyte GFAP(0.7) promoter.

This 0.7 Kb novel GFAP promoter was constructed by ligating several key regulatory elements of the 2.2Kb human GFAP promoter. It drives essentially the same expression pattern as the parental 2.2 Kb GFAP promoter but with two-fold greater activity.

Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 for mammalian expression. Wild-type ChR2, as well as a few of its mutations, provides the fastest excitation of the channelrhodopsins offered, and is widely used in in optogenetics techniques in neuroscience. hChR2(E123A) mutant accelerates the open state decay but reduces photocurrent amplitudes. Its maximal spectrum is near 485nM