AAV with GFAP(2.2) promoter driven Mito-roGFP1

This AAV expresses Mito-roGFP1 driven by an astrocyte GFAP(2.2) promoter.

The GFAP(2.2) promoter is based on human glial fibrillary acidic protein gene (GFAP), which is the major intermediate filament protein in astrocytes. It has been shown that approximately 2 kb of 5'-flanking DNA of the human GFAP gene is sufficient to direct transgene expression to astrocytes and confer developmental/injury-induced regulation.

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.